Anti-PDGF Receptor alpha 抗体 (ab96806)

製品の概要

  • 製品名Anti-PDGF Receptor alpha antibody
    PDGF Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDGF Receptor alpha
  • アプリケーション適用あり: WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human PDGF Receptor alpha.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Caco 2; SW480; MCF7; Jurkat; HepG2; NIH3T3; PC12.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 mg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 組織特異性Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 関連疾患Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Anti-PDGF Receptor alpha antibody 画像

  • All lanes : Anti-PDGF Receptor alpha antibody (ab96806) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 123 kDa
    Observed band size : 123 kDa
    Additional bands at : 32 kDa,40 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • All lanes : Anti-PDGF Receptor alpha antibody (ab96806) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 123 kDa
    Observed band size : 123 kDa
    Additional bands at : 35 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

Anti-PDGF Receptor alpha antibody (ab96806) 使用論文

ab96806 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Small intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Small intestine
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 13 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embryonic lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Embryonic lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 13 2016

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7,5% gel)
Sample Mouse Cell lysate - whole cell (OliNeu)
Specification OliNeu
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 28 2014

Thank you for your reply.

No problem to wait until October to decide on an appropriate resolution.
My colleagues from the lab are currently investigating the issue with ab96806.

Have a nice holiday.

Thank you for contacting us.

I am sorry to hear this antibody, like ab61219, is not providing clear and satisfactory results.

According to SwissProt (http://www.uniprot.org/uniprot/P16234), PDGF Receptor alpha has 3 isoforms, their si...

Read More

Thank you for your email.

I have issued a free of charge replacement with the alternative antibody ab96806 with the order number xx. The antibody should arrive tomorrow at your place. To check the status of the order please contact our Custo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Feb 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"