製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 19015157
WB 1/50 - 1/100. Detects a band of approximately 130 kDa (predicted molecular weight: 123 kDa).

ターゲット情報

  • 機能Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 組織特異性Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 関連疾患Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Anti-PDGF Receptor alpha antibody 画像

  • IHC image of ab65258 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65258, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-PDGF Receptor alpha antibody (ab65258) at 1/50 dilution

    Lane 1 : 293 cell lysate, nontransfected
    Lane 2 : 293 cell lysate, transiently transfected with the PDGFRA gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 123 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Anti-PDGF Receptor alpha antibody (ab65258) 使用論文

This product has been referenced in:
  • Francis GJ  et al. Intranasal insulin prevents cognitive decline, cerebral atrophy and white matter changes in murine type I diabetic encephalopathy. Brain 131:3311-34 (2008). IHC-P ; Mouse . Read more (PubMed: 19015157) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (kidney)
Permeabilization Yes - 1% Triton X-100 in TBS
Specification kidney
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Jun 02 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% Triton X100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Mar 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"