Anti-PDGF Receptor alpha 抗体 (ab124392)

製品の概要

  • 製品名
    Anti-PDGF Receptor alpha antibody
    PDGF Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDGF Receptor alpha
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human PDGF Receptor alpha aa 1035-1053 conjugated to keyhole limpet haemocyanin.
    Sequence:

    cGKRNRHSSQTSEESAIETG


    Database link: P16234

  • ポジティブ・コントロール
    • IHC-P: Human brain cerebellum tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/500. Detects a band of approximately 150,170 kDa (predicted molecular weight: 122 kDa).
IP Use at an assay dependent concentration. 5-10 µl immunoprecipitates PDGF Receptor alpha from 500 µg of NIH/3T3 RIPA lysate.
IHC-P 1/100.

ターゲット情報

  • 機能
    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • 組織特異性
    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • 関連疾患
    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

画像

  • ab124392, at 1/100 dilution, staining PDGF Receptor alpha in formalin-fixed, paraffin-embedded human brain cerebellum tissue by Immunohistochemistry.

参考文献

ab124392 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sarcophilus harrisii Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Username

Dr. Rachel Owen

Verified customer

投稿 Jan 16 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Juliana Schneider

Verified customer

投稿 Feb 20 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formalin-fixed paraffin-embedded sections
Username

Abcam user community

Verified customer

投稿 Aug 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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