製品の概要

  • 製品名Anti-PDGF B antibody
    PDGF B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDGF B
  • 特異性This antibody has been tested against PDGF BB protein (ab9706) and has yet to be tested against endogenous protein.
  • アプリケーション適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PDGF BB.

    (Peptide available as ab24035.)

  • ポジティブ・コントロール

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14-18 kDa).

The predicted molecular weight of PDGF BB is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF BB runs between 14-18 kDa on a gel.

IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-PDGF B antibody 画像

  • ab23914 staining PDGF BB (green) in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/500 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.

    See Abreview

  • ab23914 staining PDGF BB (green) in Mouse skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 8. Samples were incubated with primary antibody (1/250 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - WGA membranes, Blue - DAPI Nuclei.

    See Abreview

  • ab23914 staining PDGF BB in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/150) for 14 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/5000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-PDGF B antibody (ab23914) at 1 µg/ml

    Lane 1 : Recombinant PDGF BB protein (ab9706)
    Lane 2 : Recombinant PDGF BB protein (ab9706) with Human PDGF BB peptide (ab24035) at 1 µg

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 14-18 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)

    ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF BB (ab9706). The predicted molecular weight for PDGF BB is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.

  • Image courtesy of Human Protein Atlas

    ab23914 staining PDGF BB in Human kidney (male). The paraffin embedded  tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray con

  • IHC image of PDGF BB staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14-18 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14-18 kDa


    Exposure time : 1 minute

Anti-PDGF B antibody (ab23914) 使用論文

This product has been referenced in:
  • Zhang J  et al. Effects of p75 neurotrophin receptor on regulating hypoxia-induced angiogenic factors in retinal pigment epithelial cells. Mol Cell Biochem 398:123-34 (2015). WB ; Human . Read more (PubMed: 25200140) »
  • Niu F  et al. Tat 101-mediated enhancement of brain pericyte migration involves platelet-derived growth factor subunit B homodimer: implications for human immunodeficiency virus-associated neurocognitive disorders. J Neurosci 34:11812-25 (2014). WB . Read more (PubMed: 25164676) »

See all 4 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Retina)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Retina
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Aug 01 2016

Abreviews
Application Western blot
Sample Rat Recombinant protein (rat recombinant PDGF (Peprotech))
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification rat recombinant PDGF (Peprotech)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
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投稿 Jun 16 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Tris (pH9) better than Citrate (pH6)
Sample Mouse Tissue sections (Skeletal Muscle)
Specification Skeletal Muscle
Permeabilization No
Fixative Formaldehyde
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Dr. Lauren Carpenter

Verified customer

投稿 Jan 29 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris (pH8) better than Citrate (pH6)
Sample Mouse Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Permeabilization No
Fixative Methacarn
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投稿 Nov 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Permeabilization No
Fixative Formaldehyde
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投稿 Aug 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris (pH8) better than Citrate (pH6)
Sample Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Permeabilization No
Fixative Methacarn
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Abcam user community

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投稿 Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Aug 09 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (prostate cancer xenograft)
Specification prostate cancer xenograft
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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投稿 Apr 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"