Recognizes non-reduced human PDGF AA, AB and BB dimers in western immunoblots. Porcine and bovine PDGF may be detected with decreased sensitivity. Rodent PDGF will not be detected. This antibody will neutralize human, porcine, bovine and rodent PDGF.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Neut: Use at a concentration of 10 - 20 µg/ml. In a 5 day assay, 10-20ug/ml inhibited 3T3 cell growth by 60-75% as stimulated by 10ng/ml of PDGF-AB.
WB: Use at a concentration of 5 - 10 µg/ml. Predicted molecular weight: 124 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
Chen XW et al. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis. OncogeneN/A:N/A (2017).
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Song CH et al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol85:11069-78 (2011).
Read more (PubMed: 21813601) »