製品の概要

  • 製品名Anti-PDGF AA antibody
    PDGF AA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDGF AA
  • アプリケーション適用あり: WB, ELISA, Neutralising, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hPDGF-AA (human Platelet Derived Growth Factor)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPDGF-AA by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-AA.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (10.0 ng/ml), a concentration of 0.17 - 0.19 µg/ml of this antibody is required.
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
  • 配列類似性Belongs to the PDGF/VEGF growth factor family.
  • ドメインThe long form contains a basic insert which acts as a cell retention signal.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PDGF A antibody
    • PDGF A chain antibody
    • PDGF subunit A antibody
    • PDGF-1 antibody
    • PDGF1 antibody
    • PDGFA antibody
    • PDGFA_HUMAN antibody
    • Platelet derived growth factor alpha chain antibody
    • Platelet derived growth factor alpha isoform 2 preproprotein antibody
    • Platelet derived growth factor alpha polypeptide antibody
    • Platelet-derived growth factor A chain antibody
    • Platelet-derived growth factor alpha polypeptide antibody
    • Platelet-derived growth factor subunit A antibody
    see all

Anti-PDGF AA antibody 画像

  • ab9701 at 1.0 µg/ml staining normal human skin by IHC-P. The antibody was incubated overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.

Anti-PDGF AA antibody (ab9701) 使用論文

ab9701 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. Since the antibody has not been tested for cross-reactivity with mouse, we cannot guarantee that it will work. We do guarantee that an9701 will work with human samples in the following applications: Western blot, ELISA, Neutra...

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Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. The immunogen used was recombinant hPDGF-AA so I am unable to check the homolgy. You may be ab...

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To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product. I would expect that this antibody would work in this application as it was raise...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"