製品の概要

  • 製品名Anti-PDE8A antibody
    PDE8A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PDE8A
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment: RLPQGQKTAA LPRTRGAGLL ESELRDGSGK KVAVADVQFG PMRFHQDQLQ VLLVFTKEDN QCNGFCRACE KAGFKCTVTK EAQAVLACFL, corresponding to amino acids 32-121 of human PDE8A (NP_002596) with a 26 kDa tag.

  • ポジティブ・コントロール
    • PDE8A transfected 293T cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 93 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
  • 組織特異性Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
  • パスウェイPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • ドメインComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5''-cyclic phosphodiesterase 8A antibody
    • cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
    • FLJ16150 antibody
    • High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody
    • High affinity cAMP-specific and IBMX-insensitive 3'' antibody
    • HsT19550 antibody
    • PDE 8A antibody
    • PDE8A antibody
    • PDE8A_HUMAN antibody
    • Phosphodiesterase 8A antibody
    • Phosphodiesterase8A antibody
    • Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody
    see all

Anti-PDE8A antibody 画像

  • Anti-PDE8A antibody (ab77181) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 93 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PDE8A antibody (ab77181) at 5 µg/ml

    Lane 1 : PDE8A transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 93 kDa
    Observed band size : 93 kDa

Anti-PDE8A antibody (ab77181) 使用論文

ab77181 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77181.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"