製品の概要

  • 製品名Anti-PDE5A antibody
    PDE5A 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PDE5A
  • 特異性This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074; NP_236914; NP_246273). [NP_237223 has been retracted].
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-LINGESG QAKRN

    , corresponding to C terminal amino acids 864-875 of Human PDE5A.

  • ポジティブ・コントロール
    • Human lung lysate. Lysate prepared from transfected HEK293 cells transiently expressing PDE5A.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab28761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with PDE5A peptide (864-875) (ab45684).

ターゲット情報

  • 機能Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
  • 組織特異性Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
  • パスウェイPurine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • 配列類似性Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • ドメインComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
  • 翻訳後修飾Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5''-cyclic phosphodiesterase antibody
    • CGB PDE antibody
    • CGB-PDE antibody
    • CGBPDE antibody
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific phosphodiesterase antibody
    • cGMP binding/cGMP specific phosphodiesterase antibody
    • cGMP specific 3'5' cyclic phosphodiesterase antibody
    • cGMP specific phosphodiesterase PDE5A2 antibody
    • cGMP specific phosphodiesterase type 5A antibody
    • cGMP-binding cGMP-specific phosphodiesterase antibody
    • cGMP-specific 3'' antibody
    • CN5A antibody
    • CN5N antibody
    • PDE 5 antibody
    • PDE 5A antibody
    • PDE5 antibody
    • Pde5a antibody
    • PDE5A_HUMAN antibody
    • PDE5A1 antibody
    • Phosphodiesterase 5A antibody
    • Phosphodiesterase 5A cGMP specific antibody
    • Phosphodiesterase isozyme 5 antibody
    see all

Anti-PDE5A antibody 画像

  • Anti-PDE5A antibody (ab28761) at 0.3 µg/ml + Human lung lysate 30µg total protein per lane

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
  • Anti-PDE5A antibody (ab28761) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing PDE5A (isoform 2)

    Predicted band size : 100 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

Anti-PDE5A antibody (ab28761) 使用論文

ab28761 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"