Anti-PDE4D (phospho S190) 抗体 (ab59212)


  • 製品名Anti-PDE4D (phospho S190) antibody
    PDE4D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDE4D (phospho S190)
  • 特異性ab59212 detects endogenous levels of PDE4D only when phosphorylated at serine 190.
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190 (R-E-SP-F-L).

  • ポジティブ・コントロール
    • IHC-P: Human colon carcinoma tissue WB: K562 cell extracts treated with H2O2 (100uM, 30mins).


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab59212 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab59212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 91 kDa).


  • 機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 組織特異性Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • パスウェイPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 関連疾患Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • 配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻訳後修飾Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • 細胞内局在Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Anti-PDE4D (phospho S190) antibody 画像

  • All lanes : Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilution

    Lane 1 : K562 cell extract treated with H2O2 (100uM, 30mins)
    Lane 2 : K562 cell extract treated with H2O2 (100uM, 30mins) with immunising peptide

    Predicted band size : 91 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab59212, at 1/50 dilution, staining PDE4D in paraffin embedded human colon carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

Anti-PDE4D (phospho S190) antibody (ab59212) 使用論文

ab59212 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (heart)
Loading amount 25 µg
Specification heart
Gel Running Conditions Reduced Denaturing (4-12% bis tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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投稿 Dec 21 2012

Thank you for contacting Abcam.

We talked earlier on the phone about testing ab59212 in mouse via western blot. The mouse and human PDE4D protein have 98.5% homology; thus, this antibody is eligible for testing through our AbTrial program. Det...

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