Anti-PDE4D (phospho S190) 抗体 (ab59212)
Key features and details
- Rabbit polyclonal to PDE4D (phospho S190)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PDE4D (phospho S190) antibody
PDE4D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE4D (phospho S190) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human PDE4D aa 150-250 (phospho S190). (R-E-SP-F-L).
Database link: Q08499 -
ポジティブ・コントロール
- IHC-P: Human colon carcinoma tissue WB: K562 cell extracts treated with H2O2 (100uM, 30mins).
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab59212 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59212の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
WB |
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 91 kDa).
|
特記事項 |
---|
IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 91 kDa). |
ターゲット情報
-
機能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
組織特異性
Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. -
パスウェイ
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
関連疾患
Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. -
翻訳後修飾
Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. -
細胞内局在
Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 5144 Human
- Entrez Gene: 238871 Mouse
- Entrez Gene: 24627 Rat
- Omim: 600129 Human
- SwissProt: Q08499 Human
- SwissProt: Q01063 Mouse
- SwissProt: P14270 Rat
- Unigene: 117545 Human
see all -
別名
- cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
- DKFZp686M11213 antibody
- 5''-cyclic phosphodiesterase 4D antibody
see all
画像
-
All lanes : Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilution
Lane 1 : K562 cell extract treated
with H2O2 (100uM, 30mins)
Lane 2 : K562 cell extract treated
with H2O2 (100uM, 30mins) with immunising peptide
Predicted band size: 91 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
ab59212, at 1/50 dilution, staining PDE4D in paraffin embedded human colon carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab59212 は 2 報の論文で使用されています。
- Wilson NM et al. Traumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus. Front Syst Neurosci 10:5 (2016). WB . PubMed: 26903822
- Oliva AA et al. Phosphodiesterase isoform-specific expression induced by traumatic brain injury. J Neurochem 123:1019-29 (2012). PubMed: 23057870