製品の概要

  • 製品名Anti-PDE4D antibody
    PDE4D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDE4D
  • 特異性This antibody detects endogenous levels of total PDE4D protein.
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic non phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190/53 (RESPFL).

  • ポジティブ・コントロール
    • Human colon carcinoma and K562 cell extracts treated with H2O2 Human colon carcinoma tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 91 kDa).

ターゲット情報

  • 機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 組織特異性Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • パスウェイPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 関連疾患Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • 配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻訳後修飾Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • 細胞内局在Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Anti-PDE4D antibody 画像

  • All lanes : Anti-PDE4D antibody (ab59249) at 1/500 dilution

    Lane 1 : K562 cell extracts treated with H2O2 (100uM, 30mins)
    Lane 2 : K562 cell extracts treated with H2O2 (100uM, 30mins) with immunizing peptide


    Predicted band size : 91 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab59249 at 1/50 dilution staining PDE4D in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunizing peptide.

Anti-PDE4D antibody (ab59249) 使用論文

ab59249 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"