製品の概要
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製品名Anti-PDE4B antibody
PDE4B 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PDE4B
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由来種Rabbit
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特異性This antibody detects all PDE4B proteins, including PDE4B1, PDE4B2, PDE4B3 and a 66 kDa PDE4B variant. It does not cross-react with PDE4A, PDE4C or PDE4D.
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アプリケーション適用あり: WB, ICC/IF, IP, ICC, IHC-Pmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide (N terminal).
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: 20% Glycerol, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab14611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1500. Predicted molecular weight: 107 kDa.Can be blocked with PDE4B peptide (ab169989). Detects bands of 107 kDa (PDE4B1), 78 kDa (PDE4B2), 100 kDa (PDE4B3) and 66 kDa (PDE4B4). | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | 1/350. | |
| ICC | 1/300. | |
| IHC-P | Use at an assay dependent concentration. PubMed: 19924104 |
ターゲット情報
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機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
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組織特異性Expressed in brain, heart, lung and skeletal muscle.
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パスウェイPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
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配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
- Information by UniProt
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参照データベース
- Entrez Gene: 5142 Human
- Entrez Gene: 18578 Mouse
- Entrez Gene: 24626 Rat
- Omim: 600127 Human
- SwissProt: Q07343 Human
- SwissProt: P14646 Rat
- Unigene: 198072 Human
- Unigene: 20181 Mouse
see all -
別名
- 5''-cyclic phosphodiesterase 4B antibody
- cAMP-specific 3'' antibody
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
see all
画像
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Western blot - Anti-PDE4B antibody (ab14611)This image is courtesy of an anonymous AbreviewAll lanes : Anti-PDE4B antibody (ab14611) at 1/5000 dilution
All lanes : Rat mesangial cells - whole cell lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 107 kDa
Observed band size: 106 kDa (why is the actual band size different from the predicted?)
Western blot of 3 collected lysates from untreated rat mesangial cells using ab14611.
プロトコール
参考文献
This product has been referenced in:
- Cheng H et al. siRNA-mediated silencing of phosphodiesterase 4B expression affects the production of cytokines in endotoxin-stimulated primary cultured microglia. Exp Ther Med 12:2257-2264 (2016). WB . Read more (PubMed: 27698721) »
- Ji Q et al. Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation. Mol Med Rep 13:1914-22 (2016). Read more (PubMed: 26706904) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"