製品の概要

  • 製品名Anti-PDE4B antibody
    PDE4B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDE4B
  • 特異性This antibody detects all PDE4B proteins, including PDE4B1, PDE4B2, PDE4B3 and a 66 kDa PDE4B variant. It does not cross-react with PDE4A, PDE4C or PDE4D.
  • アプリケーション適用あり: WB, ICC/IF, IP, ICC, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (N terminal).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 20% Glycerol, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab14611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1500. Predicted molecular weight: 107 kDa.Can be blocked with PDE4B peptide (ab169989). Detects bands of 107 kDa (PDE4B1), 78 kDa (PDE4B2), 100 kDa (PDE4B3) and 66 kDa (PDE4B4).
ICC/IF Use at an assay dependent concentration.
IP 1/350.
ICC 1/300.
IHC-P Use at an assay dependent concentration. PubMed: 19924104

ターゲット情報

  • 機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
  • 組織特異性Expressed in brain, heart, lung and skeletal muscle.
  • パスウェイPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5''-cyclic phosphodiesterase 4B antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
    • cAMP-specific phosphodiesterase-4 B isoform antibody
    • DKFZp686F2182 antibody
    • DPDE4 antibody
    • dunce-like phosphodiesterase E4 antibody
    • MGC126529 antibody
    • OTTHUMP00000010652 antibody
    • OTTHUMP00000010653 antibody
    • OTTHUMP00000010654 antibody
    • OTTHUMP00000010656 antibody
    • OTTHUMP00000232365 antibody
    • PDE 4B antibody
    • PDE IVB antibody
    • PDE32 antibody
    • Pde4b antibody
    • PDE4B_HUMAN antibody
    • PDE4B5 antibody
    • PDEIVB antibody
    • Phosphodiesterase 4B antibody
    • Phosphodiesterase 4B cAMP specific antibody
    • Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila) antibody
    see all

Anti-PDE4B antibody 画像

  • All lanes : Anti-PDE4B antibody (ab14611) at 1/5000 dilution

    Lane 1 : Rat mesangial cells - whole cell lysate
    Lane 2 : Rat mesangial cells - whole cell lysate
    Lane 3 : Rat mesangial cells - whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 107 kDa
    Observed band size : 106 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-PDE4B antibody (ab14611) 使用論文

This product has been referenced in:
  • Shah MS  et al. Integrated microRNA and mRNA expression profiling in a rat colon carcinogenesis model: effect of a chemo-protective diet. Physiol Genomics 43:640-54 (2011). WB ; Rat . Read more (PubMed: 21406606) »
  • Wang X  et al. Cyclic nucleotide signaling in polycystic kidney disease. Kidney Int 77:129-40 (2010). IHC-P ; Mouse, Rat . Read more (PubMed: 19924104) »

See all 3 Publications for this product

Product Wall

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements for ab14611 and ab14613with the order number 1137676.

To check the status...

Read More

Attached is our blot for PDE4B1, B2, B3 and B4 proteins, there are several papers where similar analyses were published from different labs.
I am sorry but the immunogen sequences are not available.
I hope this is helpful. Please contact us a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Mesangial Cells)
Loading amount 5 µg
Specification Mesangial Cells
Treatment 1mM epsilon aminocaproic acid for 24hr
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Sep 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"