特異性This antibody detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phophorylation site.
It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site.
It does not cross-react with PDE4B, PDE4D or any other member of the PDE family.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500. Detects a band of approximately 71 kDa (predicted molecular weight: 66 kDa). Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant).
Use at an assay dependent dilution.
機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
組織特異性Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
翻訳後修飾Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA. Proteolytically cleaved by caspase-3.
細胞内局在Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
Schaal SM et al. The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as a Neuroprotectant following Spinal Cord Injury. PLoS One7:e43634 (2012).
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Zhu B et al. Activation and induction of cyclic AMP phosphodiesterase (PDE4) in rat pulmonary microvascular endothelial cells. Biochem Pharmacol68:479-91 (2004).
Read more (PubMed: 15242814) »