製品の概要

  • 製品名Anti-PDE4A antibody
    PDE4A 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PDE4A
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human protein (isoform 4: NP_006193.1)

  • ポジティブ・コントロール
    • 293T cells transfected with PDE4A

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Predicted molecular weight: 98 kDa (isoform 1) .


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
    • 組織特異性Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
    • パスウェイPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
    • 配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
    • 翻訳後修飾Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
      Proteolytically cleaved by caspase-3.
    • 細胞内局在Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 5''-cyclic phosphodiesterase 4A antibody
      • cAMP specific phosphodiesterase antibody
      • cAMP-specific 3'' antibody
      • cAMP-specific 3',5'-cyclic phosphodiesterase 4A antibody
      • DPDE2 antibody
      • dunce like phosphodiesterase E2 antibody
      • PDE 4A antibody
      • PDE4 antibody
      • PDE46 antibody
      • PDE4A antibody
      • PDE4A_HUMAN antibody
      • PDE4A11 antibody
      • Phosphodiesterase 4A antibody
      • phosphodiesterase 4A cAMP specific antibody
      • Phosphodiesterase 4A, cAMP-specific (dunce antibody
      • phosphodiesterase E2 dunce homolog antibody
      • Phosphodiesterase E2 dunce homolog, Drosophila antibody
      • phosphodiesterase isozyme 4 antibody
      • phosphodiesterase type 4A11 antibody
      see all

    Anti-PDE4A antibody 画像

    • All lanes : Anti-PDE4A antibody (ab76853) at 1/500 dilution

      Lane 1 : PDE4A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 98 (isoform 1) kDa
      Observed band size : 74 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Anti-PDE4A antibody (ab76853) 使用論文

    ab76853 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76853.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"