Anti-PDE4A 抗体 [6B6] (ab123925)


  • 製品名Anti-PDE4A antibody [6B6]
    PDE4A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6B6] to PDE4A
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human PDE4A produced in HEK293T cells (NP_006193).

  • ポジティブ・コントロール
    • HEK293T cells transfected with pCMV6-ENTRY PDE4A cDNA; Human colon, liver and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY PDE4A.
  • 特記事項Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.17% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab123925 is purified from Mouse ascites fluid by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名6B6
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab123925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/4000. Predicted molecular weight: 98 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.


  • 機能Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • 組織特異性Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • パスウェイPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • 配列類似性Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • 翻訳後修飾Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • 細胞内局在Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5''-cyclic phosphodiesterase 4A antibody
    • cAMP specific phosphodiesterase antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A antibody
    • DPDE2 antibody
    • dunce like phosphodiesterase E2 antibody
    • PDE 4A antibody
    • PDE4 antibody
    • PDE46 antibody
    • PDE4A antibody
    • PDE4A_HUMAN antibody
    • PDE4A11 antibody
    • Phosphodiesterase 4A antibody
    • phosphodiesterase 4A cAMP specific antibody
    • Phosphodiesterase 4A, cAMP-specific (dunce antibody
    • phosphodiesterase E2 dunce homolog antibody
    • Phosphodiesterase E2 dunce homolog, Drosophila antibody
    • phosphodiesterase isozyme 4 antibody
    • phosphodiesterase type 4A11 antibody
    see all

Anti-PDE4A antibody [6B6] 画像

  • ab123925 at 1/100 dilution staining PDE4A in HEK293T cells transfected with either pCMV6-ENTRY PDE4A (Red) or empty vector control plasmid (Blue) by flow cytometry.
  • ab123925 at 1/100 dilution staining PDE4A in COS7 cells transiently transfected with pCMV6-ENTRY PDE4A by Immunofluorescence.
  • ab123925 at 1/50 dilution staining PDE4A in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
  • ab123925 at 1/50 dilution staining PDE4A in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab123925 at 1/50 dilution staining PDE4A in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • All lanes : Anti-PDE4A antibody [6B6] (ab123925) at 1/4000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PDE4A cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 98 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC207765)

Anti-PDE4A antibody [6B6] (ab123925) 使用論文

ab123925 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I have been in contact with the lab about this product, the epitope that this product recognizes is not mapped, so we don't know if it would recognize all the isoforms of PDE4A.
I would also like to inform you...

Read More