製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PDE11A
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    C-NSFKESMEKSSYSD

    , corresponding to internal sequence amino acids 455-468 of Human PDE11A (NP_058649.3; NP_001070665.1; NP_001070826.1; NP_001070664.1)

  • ポジティブ・コントロール
    • Human prostate tissue; Human prostate lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/2000.
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 105 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.
  • 組織特異性
    Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil (Cialis) is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed in adrenal cortex.
  • 関連疾患
    Defects in PDE11A are the cause of primary pigmented nodular adrenocortical disease type 2 (PPNAD2) [MIM:610475]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. PPNAD2 is characterized by adrenal glands with overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. PPNAD2 leads to Cushing syndrome.
  • 配列類似性
    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • ドメイン
    The tandem GAF domains bind cGMP, and regulate enzyme activity. The binding of cGMP leading to stimulate the enzyme activity.
  • 細胞内局在
    Cytoplasm > cytosol.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5''-cyclic-AMP and -GMP phosphodiesterase 11A antibody
    • cAMP and cGMP phosphodiesterase 11A antibody
    • cGMP phosphodiesterase 11A antibody
    • Cyclic nucleotide phosphodiesterase 11A antibody
    • Dual 3'' antibody
    • Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A antibody
    • pde11 antibody
    • PDE11_HUMAN antibody
    • pde11a antibody
    • PDE11A1 antibody
    • pde11a2 antibody
    • Phosphodiesterase 11A antibody
    • PPNAD2 antibody
    see all

画像

  • ab115624, at 10 µg/ml, staining PDE11A in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-PDE11A antibody (ab115624) at 1 µg/ml + Human prostate lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 105 kDa

参考文献

ab115624 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab115624.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録