製品の概要

  • 製品名
    Anti-Pdd1 antibody - ChIP Grade
  • 製品の詳細
    Rabbit polyclonal to Pdd1 - ChIP Grade
  • アプリケーション
    適用あり: ICC/IF, WB, IP, ChIPmore details
  • 種交差性
    交差種: Tetrahymena
  • 免疫原

    Synthetic peptide derived from residues 300 - 400 of Tetrahymena sp. Pdd1.

    .

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa).
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration. PubMed: 20357003

ターゲット情報

  • 関連性
    The PDD1 gene sequence predicts a novel polypeptide containing two copies of a chromodomain, a conserved domain found in a number of heterochromatin-associated proteins (Koonin et al. 1995). Consistent with this finding, Pdd1p localizes to electron-dense heterochromatic structures that concentrate at the periphery of macronuclear anlagen during the time when DNA deletion occurs.
  • 細胞内局在
    Nuclear
  • 参照データベース
    • 別名
      • p65 antibody
      • Pdd 1 antibody
      • Pdd 1p antibody
      • Pdd1p antibody
      • Programmed DNA degradation (pdd) antibody
      see all

    画像

    • ab5338 staining Pdd1 in wild-type Tetrahymena sp. cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol. The primary antibody was diluted 1/250 in TBS + 1% Goat Serum and incubated with the sample for 18 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.

      See Abreview

    • All lanes : Anti-Pdd1 antibody - ChIP Grade (ab5338) at 1/1000 dilution

      Lane 1 : Wild-type Tetrahymena sp. Starved Tetrahymena, do not express pdd1p (hour 0)
      Lane 2 : Wild-type Tetrahymena sp. Conjugating Tetrahymena, peak of pdd1p expression (hour 9)

      Lysates/proteins at 15000 cells per lane.

      Secondary
      HRP-conjugated Goat anti-Rabbit at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 64 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      This image is courtesy of an anonymous Abreview.

      See Abreview

    参考文献

    This product has been referenced in:
    • Suhren JH  et al. Negative Regulators of an RNAi-Heterochromatin Positive Feedback Loop Safeguard Somatic Genome Integrity in Tetrahymena. Cell Rep 18:2494-2507 (2017). Read more (PubMed: 28273462) »
    • Kataoka K  et al. Phosphorylation of an HP1-like protein is a prerequisite for heterochromatin body formation in Tetrahymena DNA elimination. Proc Natl Acad Sci U S A 113:9027-32 (2016). IF . Read more (PubMed: 27466409) »

    See all 12 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Tetrahymena sp. Cell lysate - whole cell (wildtype)
    Loading amount
    15000 cells
    Specification
    wildtype
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 22 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Tetrahymena sp. Cell (wildtype)
    Specification
    wildtype
    Fixative
    Methanol
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    投稿 Sep 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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