Anti-PDCD5 抗体 (ab75430)
Key features and details
- Rabbit polyclonal to PDCD5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDCD5 antibody
PDCD5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDCD5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human PDCD5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90255) -
ポジティブ・コントロール
- Recombinant Human PDCD5 protein (ab78938) can be used as a positive control in WB. This antibody gave a positive signal in HEK293 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75430の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
May function in the process of apoptosis. -
組織特異性
Widely expressed. Highest levels in heart, testis, kidney, pituitary gland, adrenal gland and placenta. -
配列類似性
Belongs to the PDCD5 family. -
発生段階
Expression in fetal tissues is significantly lower than in adult tissues. - Information by UniProt
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参照データベース
- Entrez Gene: 9141 Human
- Entrez Gene: 100042424 Mouse
- Entrez Gene: 56330 Mouse
- Entrez Gene: 292814 Rat
- Omim: 604583 Human
- SwissProt: O14737 Human
- SwissProt: P56812 Mouse
- Unigene: 443831 Human
see all -
別名
- PDCD5 antibody
- PDCD5_HUMAN antibody
- Programmed cell death protein 5 antibody
see all
画像
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Anti-PDCD5 antibody (ab75430) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 100 kDa, 52 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
ICC/IF image of ab75430 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of ab75430 staining in Human Colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75430, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab75430 は 2 報の論文で使用されています。
- Zhao H et al. PDCD5 inhibits osteosarcoma cell metastasis via targeting TGF-ß1/Smad signaling pathway and is associated with good prognosis. Am J Transl Res 11:1116-1128 (2019). PubMed: 30899411
- Gao F et al. Clinicopathological Significance of Decreased Expression of the Tumor Inhibitor Gene PDCD5 in Osteoclastoma. Genet Test Mol Biomarkers 23:807-814 (2019). PubMed: 31638427