Anti-PDCD4 (phospho S457) 抗体 (ab118505)

製品の概要

  • 製品名
    Anti-PDCD4 (phospho S457) antibody
    PDCD4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PDCD4 (phospho S457)
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Orangutan
  • 免疫原

    Synthetic phosphopeptide: CPSRGRKRFVSPEGDGGRLK conjugated to KLH, corresponding to amino acids 447-465 of Human PDCD4

  • ポジティブ・コントロール
    • Human breast tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/5000. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 1.25 - 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1500 - 1/7000.

ターゲット情報

  • 機能
    Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • 組織特異性
    Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • 配列類似性
    Belongs to the PDCD4 family.
    Contains 2 MI domains.
  • ドメイン
    Binds EIF4A1 via both MI domains.
  • 翻訳後修飾
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • 細胞内局在
    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

画像

  • ab118505, at 2.5 µg/ml, staining PDCD4 in formalin-fixed, paraffin-embedded Human Breast tissue by Immunohistochemistry using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

参考文献

ab118505 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab118505.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録