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RabMAb

Anti-PDCD4 抗体 [EPR3432] (ab79405)

製品の概要

  • 製品名
    Anti-PDCD4 antibody [EPR3432]
    PDCD4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3432] to PDCD4
  • アプリケーション
    適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human PDCD4

  • ポジティブ・コントロール
    • Jurkat and HeLa cell lysates; human breast carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-PDCD4 antibody (Phycoerythrin) [EPR3432] (ab209301)

    Anti-PDCD4 antibody (Alexa Fluor® 405) [EPR3432] (ab210146)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
    • 組織特異性
      Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
    • 配列類似性
      Belongs to the PDCD4 family.
      Contains 2 MI domains.
    • ドメイン
      Binds EIF4A1 via both MI domains.
    • 翻訳後修飾
      Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
    • 細胞内局在
      Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Death up-regulated gene protein antibody
      • Dug antibody
      • H731 antibody
      • Ma3 antibody
      • MGC33046 antibody
      • MGC33047 antibody
      • Neoplastic transformation inhibitor antibody
      • Neoplastic transformation inhibitor protein antibody
      • Nuclear antigen H731 antibody
      • Nuclear antigen H731 like antibody
      • Nuclear antigen H731 like protein antibody
      • Nuclear antigen H731-like antibody
      • PDCD 4 antibody
      • Pdcd4 antibody
      • PDCD4_HUMAN antibody
      • Programmed cell death 4 antibody
      • programmed cell death 4 (neoplastic transformation inhibitor) antibody
      • Programmed cell death protein 4 antibody
      • Protein 197/15a antibody
      • Protein MA-3 antibody
      • Tis antibody
      • Topoisomerase-inhibitor suppressed protein antibody
      see all

    画像

    • All lanes : Anti-PDCD4 antibody [EPR3432] (ab79405) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
      Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
    • ab79405 at 1/1000 dilution staining PDCD4 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.

    参考文献

    This product has been referenced in:
    • Liang X  et al. MicroRNA-16 suppresses the activation of inflammatory macrophages in atherosclerosis by targeting PDCD4. Int J Mol Med 37:967-75 (2016). Read more (PubMed: 26936421) »
    • Wright EC  et al. MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs. Reprod Biol Endocrinol 14:21 (2016). Read more (PubMed: 27084064) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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