The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/250 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Belongs to the PDCD4 family. Contains 2 MI domains.
Binds EIF4A1 via both MI domains.
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
Neoplastic transformation inhibitor protein antibody
Nuclear antigen H731 antibody
Nuclear antigen H731 like antibody
Nuclear antigen H731 like protein antibody
Nuclear antigen H731-like antibody
PDCD 4 antibody
Programmed cell death 4 antibody
programmed cell death 4 (neoplastic transformation inhibitor) antibody
Programmed cell death protein 4 antibody
Protein 197/15a antibody
Protein MA-3 antibody
Topoisomerase-inhibitor suppressed protein antibody
Western blot - Anti-PDCD4 antibody [EPR3432] (ab79405)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: PDCD4 knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab79405 observed at 52 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab79405 was shown to recognize PDCD4 in wild-type HAP1 cells as signal was lost at the expected MW in PDCD4 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDCD4 knockout samples were subjected to SDS-PAGE. ab79405 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - PDCD4 antibody [EPR3432] (ab79405)
All lanes : Anti-PDCD4 antibody [EPR3432] (ab79405) at 1/10000 dilution
Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa Observed band size: 52 kDa Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.