The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 105-8 cells.
Use 0.5µg for 105-8 cells in 100µl.
ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Possible cell death inducer, in association with other factors.
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 µg of FITC Rat IgG2b isotype control (open histogram), or 0.25 µg of ab93579
(colored histogram). Total viable cells were used for analysis.