製品の概要

  • 製品名
    Anti-PD1 antibody [RMP1-14]
    PD1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RMP1-14] to PD1
  • アプリケーション
    適用あり: IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    PDCD1 transfected BHK cells.

  • 特記事項


    Antibodies produced by clone RMP1-14 have been reported to block the binding of both B7-H1-Ig and B7-DC-Ig fusion proteins to PDCD1 transfected BHK cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.

Formaldehyde-based fixatives such as PFA are not recommended. Fix frozen, unfixed tissue sections on the slide with ice-cold acetone for 15-20 minutes.

Flow Cyt 1/10 - 1/25. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Possible cell death inducer, in association with other factors.
  • 関連疾患
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 配列類似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 発生段階
    Induced at programmed cell death.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

Anti-PD1 antibody [RMP1-14] (ab63477) 使用論文

This product has been referenced in:
  • Gray MJ  et al. Phosphatidylserine-targeting antibodies augment the anti-tumorigenic activity of anti-PD-1 therapy by enhancing immune activation and downregulating pro-oncogenic factors induced by T-cell checkpoint inhibition in murine triple-negative breast cancers. Breast Cancer Res 18:50 (2016). Read more (PubMed: 27169467) »
  • Ehlers M  et al. Immunoregulatory natural killer cells suppress autoimmunity by down-regulating antigen-specific CD8+ T cells in mice. Endocrinology 153:4367-79 (2012). Read more (PubMed: 22733969) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (lymphocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jan 05 2017

The amino acid sequence of the antibody itself has not been determined, to our knowledge.

If you need the sequence of the immunogen that generated the antibody, the immunogen and immunization procedure are described in a paper by the laborator...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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