製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50. Predicted molecular weight: 32 kDa.
Flow Cyt Use at an assay dependent concentration.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
IP Use at an assay dependent concentration.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Possible cell death inducer, in association with other factors.
  • 関連疾患Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 発生段階Induced at programmed cell death.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

Anti-PD1 antibody [NAT105] 画像

  • Double immunofluorescence staining of CD3 (green) and PD1 (red) on paraffin embedded tonsil.
  • Paraffin-embedded human tonsil section stained with ab52587 PD1 antibody at 1/50 dilution.
  • Immunohistochemical analysis of frozen Human liver tissue labeling PD1 with ab52587 at 1/50 dilution.

    See Abreview

  • Anti-PD1 antibody [NAT105] (ab52587) at 1/50 dilution + YT cell line extracts

    Predicted band size : 32 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous collaborator.

  • Double immunoenzymatic staining of Ki67 (brown) and PD1 (red) on paraffin embedded tonsil.
  • Sample: Human tonsil cell extract
    Dilution: ab52587 antibody was used as 1:200 in 1x106 cells/tube.
    Anti-CD4 antibody was used as 1:200 dilution
    Anti-CD8 antibody was used as 1:200 dilution
  • Tissue Sample: Human Tonsil
    ab52587 diluted 1:100
    Incubation time: 30 mins

Anti-PD1 antibody [NAT105] (ab52587) 使用論文

This product has been referenced in:
  • Kammerer-Jacquet SF  et al. Independent association of PD-L1 expression with noninactivated VHL clear cell renal cell carcinoma-A finding with therapeutic potential. Int J Cancer 140:142-148 (2017). Read more (PubMed: 27623354) »
  • Bastman JJ  et al. Tumor-infiltrating T Cells and the PD-1 Checkpoint Pathway in Advanced Differentiated and Anaplastic Thyroid Cancer. J Clin Endocrinol Metab N/A:jc20154227 (2016). IHC ; Human . Read more (PubMed: 27045886) »

See all 22 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Leica ER2
Sample Monkey Tissue sections (Lymphoid tisssues - spleen, tonsil and lymph node)
Specification Lymphoid tisssues - spleen, tonsil and lymph node
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Acetone/Methanol 1:2
Permeabilization Yes - TBS + 0,1% TritonX100
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Follicular Lymphoma, Lymph node)
Specification Follicular Lymphoma, Lymph node
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6
Blocking step Novolink Protein Block, Novocastra, Leica Microsystems Inc. as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Hajnalka Rajnai

Verified customer

投稿 Nov 30 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Low pH buffer
Permeabilization No
Specification Human lymph node
Blocking step Dako endogenouse peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate Cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Specification Prostate Cancer
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Low pH
Sample Human Tissue sections (Malignant Melanoma)
Specification Malignant Melanoma
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 06 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer ph 8,5
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Oct 22 2014

.

You may want to consider the following secondary antibodies:

http://www.abcam.com/donkey-fab2-polyclonal-secondary-antibody-to-mouse-igg-hl-alexa-fluorreg-488-pre-adsorbed-ab150101.html

http://www.abcam.com/donkey-fab2-poly...

Read More

I can confirm that the epitope of ab52587 is extracellular, because in FACS experiments fresh, unfixed cells were used.

I can suggest the following secondary antibodies:
goat anti-mouse antibody
http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html
or
the rabbit anti-mouse antibody
http://www....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"