Anti-PD1 抗体 [NAT105] (ab52587)
Key features and details
- Mouse monoclonal [NAT105] to PD1
- Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PD1 antibody [NAT105]
PD1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [NAT105] to PD1 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, IHC-Fr, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human tonsil tissue. ICC/IF: MOLT-4 cells. Flow Cyt: Human tonsil extract. MOLT-4 cells. IHC-Fr: Human tonsil tissue.
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特記事項
Please note that PD-1 is expressed variably in different tissues and that optimisation may be required depending on the tissue used for the experiment.
Western blot protocol advice:
Due to low expression of PD-1, we recommend loading a high amount of sample (100 µg) to detect the band for PD-1. Human tonsil and YT cell line lysates are suitable positive controls.
New alternative versions of the NAT105 clone available:
Recombinant version (ab234444)
PBS-only recombinant version (ab201811)
Chimeric recombinant rabbit version (ab216352)To test multiple PD-1 monoclonal antibodies please see our PD-1 trial-size panel – ab252192
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
NAT105 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- Anti-PD1 antibody [NAT105] - BSA and Azide free (ab201811)
- Anti-PD1 antibody [NAT105] - Rabbit IgG (Chimeric) (ab216352)
- Alexa Fluor® 488 Anti-PD1 antibody [NAT105] (ab220300)
- Alexa Fluor® 647 Anti-PD1 antibody [NAT105] (ab220301)
- PE Anti-PD1 antibody [NAT105] (ab220302)
- Anti-PD1 antibody [NAT105] (ab234444)
- Alexa Fluor® 555 Anti-PD1 antibody [NAT105] (ab280864)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52587の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50. Predicted molecular weight: 32 kDa.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 - 10 µg/ml.
We recommend Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody. |
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IHC-P | (7) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/50. Predicted molecular weight: 32 kDa. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 - 10 µg/ml. We recommend Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody. |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Possible cell death inducer, in association with other factors. -
関連疾患
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
発生段階
Induced at programmed cell death. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5133 Human
- Omim: 600244 Human
- SwissProt: Q15116 Human
- Unigene: 158297 Human
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別名
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
画像
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ab52587 staining PD1 in MOLT4 treated with Ionomycin (500 ng/ml, 24 h) and PMA (10 ng/ml, 24 h) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab52587 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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IHC image of PD1 staining in normal human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52587 at 5 µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Double immunofluorescence staining of CD3 (green) and PD1 (red) on paraffin embedded tonsil.
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Sample: Human tonsil cell extract.
Dilution: ab52587 antibody was used as 1/200 in 1x106 cells/tube.
Anti-CD4 antibody was used as 1/200 dilution.
Anti-CD8 antibody was used as 1/200 dilution. -
Formaldehyde-fixed, paraffin-embedded human follicular lymphoma tissue stained for PD1 with ab52587 at 1/100 dilution in immunohistochemical analysis.
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Anti-PD1 antibody [NAT105] (ab52587) at 1/50 dilution + YT cell line extracts
Predicted band size: 32 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
Representative immunohistochemical staining results for PD1 using ab52587 at a 1/50 dilution in human formalin-fixed, paraffin-embedded tissue specimens.
Panel A: Normal lung tissue, negative control;
Panel B: Tonsillar tissue, positive control;
Panel C: PD1-negative tumor infiltrating lymphocytes;
Panel D: PD1-positive tumor infiltrating lymphocytes in squamous cell carcinomas).
Image from PMID: 26313362
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Immunohistochemical analysis of various soft tissue sarcomas staining PD1 using ab52587 at a 1/50 dilution.
Arrows indicate PD1 positive lymphocytes.
Image from PMID: 24349382
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Overlay histogram showing MOLT-4 (Human lymphoblastic leukemia cell line) cells stained with ab52587 (red line). Live cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab52587, 1/100 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 4°C.
A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (black line). Unlabeled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. -
Immunohistochemical analysis of human large and locally advanced breast cancers staining PD1 using ab52587.
(Panel a) Low level of PD-1+ T cell infiltration.
(Panel b) high level of PD-1+ T cell infiltration. (Itu: intratumoral Str: stromal).
Image data from PMID: 27777963
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Double immunoenzymatic staining of Ki67 (brown) and PD1 (red) on paraffin embedded tonsil.
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Human tonsil tissue stained for PD1 with ab52587 incubated for 30 mins at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemical analysis of frozen human liver tissue labeling PD1 with ab52587 at 1/50 dilution.
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Expression of markers of T cell differentiation and degree of inflammation in the heart of chronically T. cruzi-infected subjects with severe cardiomyopathy.
A–D, left panel: representative photos of CD45RO, CD27, PD1 and CD57 expression, respectively.
Panel C shown.
Image data from PMID: 25144227
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (255)
ab52587 は 255 報の論文で使用されています。
- Schwarze JK et al. Low-Dose Nivolumab with or without Ipilimumab as Adjuvant Therapy Following the Resection of Melanoma Metastases: A Sequential Dual Cohort Phase II Clinical Trial. Cancers (Basel) 14:N/A (2022). PubMed: 35158952
- Nan L et al. ARID1A Downregulation Predicts High PD-L1 Expression and Worse Clinical Outcome in Patients With Gallbladder Cancer. Front Oncol 12:787897 (2022). PubMed: 35198440
- Johnson BE et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med 3:100525 (2022). PubMed: 35243422
- Cao Y et al. Lymphocytes Infiltration and Expression of PD-1 and PD-L1 in Colorectal Cancer Between HIV-Infected and Non-HIV-Infected Patients: A Propensity Score Matched Cohort Study. Front Oncol 12:827596 (2022). PubMed: 35311077
- Yang H et al. SIRPα and PD1 expression on tumor-associated macrophage predict prognosis of intrahepatic cholangiocarcinoma. J Transl Med 20:140 (2022). PubMed: 35317832