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Syrian Hamster kidney cell line BKH transfected with Mouse PD1 cDNA.
It is reported that monoclonal antibody J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.
Our Abpromise guarantee covers the use of ab95789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.25-0.5µg for 105 cells. Cell number should be determined empirically but can range from 105 to 108 cells/test (based on a test volume of 100 µl).
ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"