製品の概要

  • 製品名Anti-PCYT1A antibody
    PCYT1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PCYT1A
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Horse, Cow
  • 免疫原

    Recombinant fragment: DAQCSAKVNA RKRRKEAPGP NGATEEDGVP SKVQRCAVGL RQPAPFSDEI EVDFSKPYVR VTMEEASRGT PCERPVRVYA DGIFDLFHS, corresponding to amino acids 2-91 of Human PCYT1A (NP_005008) with a 26 kDa tag.

  • ポジティブ・コントロール
    • PC-12 and K-562 cell lysates. Recombinant tagged human PCYT1A fragment (immunogen).

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged PCYT1A is approximately 3 ng/ml when used as a capture antibody.

ターゲット情報

  • 機能Controls phosphatidylcholine synthesis.
  • パスウェイPhospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
  • 配列類似性Belongs to the cytidylyltransferase family.
  • 翻訳後修飾The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
  • 細胞内局在Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCT A antibody
    • CCT alpha antibody
    • CCT-alpha antibody
    • Choline phosphate cytidylyltransferase A antibody
    • Choline-phosphate cytidylyltransferase A antibody
    • CT A antibody
    • CT antibody
    • CTP:phosphocholine cytidylyltransferase A antibody
    • CTPCT antibody
    • PCY1A_HUMAN antibody
    • PCYT1 antibody
    • Pcyt1a antibody
    • Phosphate cytidylyltransferase 1 choline alpha antibody
    • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
    • Phosphorylcholine transferase A antibody
    see all

Anti-PCYT1A antibody 画像

  • Anti-PCYT1A antibody (ab77305) at 5 µg/ml + recombinant tagged human PYCT1A fragment (the immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 42 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-PCYT1A antibody (ab77305) at 5 µg/ml + PC-12 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • Anti-PCYT1A antibody (ab77305) at 5 µg/ml + K-562 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

Anti-PCYT1A antibody (ab77305) 使用論文

ab77305 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Hindlimb muscle)
Loading amount 40 µg
Specification Hindlimb muscle
Gel Running Conditions Reduced Denaturing (gradient 4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. David Russ

Verified customer

投稿 Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"