製品の概要

  • 製品名Anti-PCSK9 antibody
    PCSK9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PCSK9
  • 特異性Detects both the zymogen and active forms of PC9. Smaller cleavage products are also detected.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human PCSK9 (C terminal). From the propeptide cleavage site. Based on the catalytic domain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Predicted molecular weight: 74 kDa. 1/1000 when using colorimetric substrates and 1/5000 for chemiluminescent substrates. (pre-pro-peptide runs at 78-80 kDa, the mature form at 58 kDa)

ターゲット情報

  • 機能May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • 組織特異性Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • 関連疾患Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • 配列類似性Belongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • 翻訳後修飾The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Convertase subtilisin/kexin type 9 preproprotein antibody
    • FH3 antibody
    • HCHOLA3 antibody
    • Hypercholesterolemia autosomal dominant 3 antibody
    • LDLCQ1 antibody
    • NARC 1 antibody
    • NARC-1 antibody
    • NARC1 antibody
    • Neural apoptosis regulated convertase 1 antibody
    • Neural apoptosis-regulated convertase 1 antibody
    • PC 9 antibody
    • PC9 antibody
    • PCSK 9 antibody
    • PCSK9 antibody
    • PCSK9_HUMAN antibody
    • Proprotein convertase 9 antibody
    • Proprotein convertase PC9 antibody
    • Proprotein convertase subtilisin/kexin type 9 antibody
    • PSEC0052 antibody
    • Subtilisin/kexin like protease PC9 antibody
    • Subtilisin/kexin-like protease PC9 antibody
    see all

Anti-PCSK9 antibody 画像

  • ICC/IF image of ab52754 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52754, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PCSK9 antibody (ab52754) 使用論文

ab52754 has not yet been referenced specifically in any publications.

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