ICC/IF image of ab28770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28770, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, staining PCSK9 with ab28770 at 4 µg/ml. Staining was detected using HRP. Antigen retrieval was by heat mediation in TRIS/EDTA buffer (pH 9).
Western blot - Anti-PCSK9 antibody (ab28770)
Anti-PCSK9 antibody (ab28770) at 0.3 µg/ml + Human Adipose lysate in RIPA buffer at 35 µg