製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

ターゲット情報

  • 機能Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus.
  • 組織特異性Expressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus.
  • 配列類似性Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • 細胞内局在Cell membrane and Cytoplasm > perinuclear region. Locates to the perinuclear areas within the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ch-1PTPase antibody
    • Ch1 PTPase antibody
    • DKFZp781C1038 antibody
    • EC PTP antibody
    • ECPTP antibody
    • FLJ34328 antibody
    • MGC131968 antibody
    • MGC148170 antibody
    • NC PTPCOM1 antibody
    • NC-PTPCOM1 antibody
    • Protein tyrosine phosphatase Cr1PTPase precursor antibody
    • Protein tyrosine phosphatase NC PTPCOM1 antibody
    • Protein tyrosine phosphatase PCPTP1 antibody
    • Protein tyrosine phosphatase receptor type R antibody
    • Protein-tyrosine phosphatase PCPTP1 antibody
    • PTP SL antibody
    • PTPBR7 antibody
    • PTPRQ antibody
    • PTPRR antibody
    • PTPRR_HUMAN antibody
    • PTPSL antibody
    • R-PTP-R antibody
    • Receptor type tyrosine protein phosphatase R antibody
    • Receptor-type tyrosine-protein phosphatase R antibody
    see all

Anti-PCPTP1 antibody 画像

  • All lanes : Anti-PCPTP1 antibody (ab12153) at 0.4 µg/ml

    Lane 1 : 0.01µg PTP beta (PTP RB)
    Lane 2 : 0.01µg PTP epsilon (PTP RE)
    Lane 3 : 0.01µg LAR (PTP RF)
    Lane 4 : 0.01µg PTP gamma (PTP RG)
    Lane 5 : 0.01µg SAP1 (PTP RH)
    Lane 6 : 0.01µg DEP1 (PTP RJ)
    Lane 7 : 0.01µg PTP kappa (PTP RK)
    Lane 8 : 0.01µg PTPIA2 (PTP RN)
    Lane 9 : 0.01µg GLEPP1 (PTP RO)
    Lane 10 : 0.01µg PCPTP1 (PTP RR)
    Lane 11 : 0.01µg CD45 (PTP RC)

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/ml

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa


    Recombinant PCPTP1 phosphatase domains (0.01µg per lane) were probed with ab12153 at 0.4µg/ml

    Phosphatase domain fusion proteins provided by Purely Proteins

  • Image courtesy of Human Protein Atlas    

    ab12153 staining PCPTP1 in Human cerebellum. The paraffin embedded tissue was incubated with ab12153 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab12153 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

Anti-PCPTP1 antibody (ab12153) 使用論文

ab12153 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"