製品の概要

  • 製品名Anti-PCNA antibody [PC10], prediluted
    PCNA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PC10] to PCNA, prediluted
  • 特異性PCNA is essential for cellular DNA synthesis. This antibody reacts with proliferating cells in a wide range of normal tissues. The significance of PCNA immunoreactivity in tumors in not fully established yet. However, in non-Hodgkin's lymphoma, a linear relation between Ki-67 and PCNA staining has been demonstrated.
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Rat, Rabbit, Human
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Fusion protein corresponding to Rat PCNA.

  • ポジティブ・コントロール
    • ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Treat tissue sections with 0.4 N HCL for 10-30 minutes at RT. Although not required, microwave treatment, prior to immunostaining, considerably increases the signal.

IHC-Fr Use at an assay dependent concentration.

This antibody has been pretitered and quality controlled to work on acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Treat tissue sections with 0.4 N HCL for 10-30 minutes at RT. Although not required, microwave treatment, prior to immunostaining, considerably increases the signal.

ターゲット情報

  • 機能This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • 配列類似性Belongs to the PCNA family.
  • 翻訳後修飾Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • 細胞内局在Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Anti-PCNA antibody [PC10], prediluted 画像

  • ICC/IF image of ab912 stained HeLa cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab912, Neat) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PCNA antibody [PC10], prediluted (ab912) 使用論文

This product has been referenced in:
  • Wang Q  et al. Role of the mTOR Signalling Pathway in Experimental Rabbit Vein Grafts. Heart Lung Circ 25:1124-1132 (2016). IHC-P ; Rabbit . Read more (PubMed: 27185657) »
  • Hennings L  et al. Dead or alive? Autofluorescence distinguishes heat-fixed from viable cells. Int J Hyperthermia 25:355-63 (2009). IHC-P ; Rabbit . Read more (PubMed: 19533483) »

See all 3 Publications for this product

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Thank you for your enquiry. This antibody is designed specifically to be reacy-to-use for IHC. The concentration is approximately 1-2 ug/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Abcam can not advise on individual protocols but instead suggests that customers follow the general protocols available on the abcam homepage.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"