Anti-PCNA 抗体 [PC10 (a.k.a. 3F81)] (Phycoerythrin) (ab93576)

製品の概要

  • 製品名Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin)
    PCNA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PC10 (a.k.a. 3F81)] to PCNA (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション適用あり: IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Protein A-Rat PCNA fusion obtained from pC2T.

  • ポジティブ・コントロール
    • Alcohol fixed MOLT-4 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
Flow Cyt
  • 追加情報Flow Cyt: Use 20 µl (or 0.125 µg) for 105-8 cells in a 100 µl volume per test.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
    • 配列類似性Belongs to the PCNA family.
    • 翻訳後修飾Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
      Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
    • 細胞内局在Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATLD2 antibody
      • cb16 antibody
      • Cyclin antibody
      • DNA polymerase delta auxiliary protein antibody
      • etID36690.10 antibody
      • fa28e03 antibody
      • fb36g03 antibody
      • HGCN8729 antibody
      • MGC8367 antibody
      • Mutagen-sensitive 209 protein antibody
      • OTTHUMP00000030189 antibody
      • OTTHUMP00000030190 antibody
      • PCNA antibody
      • Pcna/cyclin antibody
      • PCNA_HUMAN antibody
      • PCNAR antibody
      • Polymerase delta accessory protein antibody
      • Proliferating cell nuclear antigen antibody
      • wu:fa28e03 antibody
      • wu:fb36g03 antibody
      see all

    Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin) 画像

    • Intracellular staining of Molt-4 cells with FITC conjugated anti-Human PCNA (PC10) (left), and ab93576 (right). Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

    Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin) (ab93576) 使用論文

    ab93576 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (4-15%)
    Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    投稿 Jan 28 2013

    Application Immunohistochemistry (Frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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    Verified customer

    投稿 Mar 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"