Anti-PCNA 抗体 (Biotin) (ab113270)


  • 製品名Anti-PCNA antibody (Biotin)
    PCNA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PCNA (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: Flow Cyt, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Alligator, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 75 and 125 of Human PCNA (UniProt P12004).

  • ポジティブ・コントロール
    • HeLa whole cell lysate





Our Abpromise guarantee covers the use of ab113270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.03µg for 106 cells. in 150 µl total volume.

ab200208-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IP Use at 2-5 µg/mg of lysate.


  • 機能This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • 配列類似性Belongs to the PCNA family.
  • 翻訳後修飾Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • 細胞内局在Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Anti-PCNA antibody (Biotin) 画像

  • All lanes : Anti-PCNA antibody (Biotin) (ab113270) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    developed using the ECL technique

    Predicted band size : 29 kDa

    Exposure time : 3 seconds

Anti-PCNA antibody (Biotin) (ab113270) 使用論文

ab113270 has not yet been referenced specifically in any publications.

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What is the unconjugated version of the biotinylated antibody ab113270?