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Our Abpromise guarantee covers the use of ab19166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19945538|
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.03µg for 106 cells.
0.03 μg per 1 million cells in a 150 mcl reaction.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).|
Immunohistochemistry (Frozen sections) analysis of 8 μm sections of mouse prostate triple labelled with rabbit polyclonal antibody for PCNA (ab19166) (1/200, green), mouse monoclonal antibody for smooth muscle actin (red) and the nuclear stain DAPI (blue). Arrows indicate positive staining for PCNA and DAPI (i.e. nuclei of proliferating cells). L, lumen; E, epithelium; S, smooth muscle stroma. Calibration bar=200 μm.
ab19166 (Human PCNA antibody) in western blot in A431 whole cell lysate:
Lane 1: 15
Lane 2: 50
Lane 3: 15
Lane 4: 50
WB exposure: 2 minutes