Anti-PCNA 抗体 [5E6/2] (ab70472)
Key features and details
- Mouse monoclonal [5E6/2] to PCNA
- Suitable for: IP, ELISA, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
製品の概要
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製品名
Anti-PCNA antibody [5E6/2]
PCNA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5E6/2] to PCNA -
由来種
Mouse -
アプリケーション
適用あり: IP, ELISA, WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein corresponding to Saccharomyces cerevisiae PCNA. Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.
Database link: P15873 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
5E6/2 -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70472の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
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特記事項 |
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IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
ターゲット情報
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機能
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
配列類似性
Belongs to the PCNA family. -
翻訳後修飾
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
細胞内局在
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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参照データベース
- Entrez Gene: 852385 Saccharomyces cerevisiae
- SwissProt: P15873 Saccharomyces cerevisiae
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別名
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab70472 は 15 報の論文で使用されています。
- Brown RE et al. The RNA export and RNA decay complexes THO and TRAMP prevent transcription-replication conflicts, DNA breaks, and CAG repeat contractions. PLoS Biol 20:e3001940 (2022). PubMed: 36574440
- Reyes GX et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair. Curr Biol 31:1268-1276.e6 (2021). PubMed: 33417883
- Guilliam TA & Yeeles JTP Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart. Nat Struct Mol Biol 27:450-460 (2020). PubMed: 32341533
- Kulkarni DS et al. PCNA activates the MutL? endonuclease to promote meiotic crossing over. Nature 586:623-627 (2020). PubMed: 32814343
- Paul Solomon Devakumar LJ et al. Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex. Nucleic Acids Res 47:6826-6841 (2019). PubMed: 31114918