製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PCMT1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-197 of Human PCMT1 (NP_005380)

  • ポジティブ・コントロール
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IHC-P: U87 xenograft

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 関連性
    Function: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Similarity: Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • L isoaspartyl protein carboxyl methyltransferase antibody
    • L isoaspartyl/D aspartyl methyltransferase antibody
    • L-isoaspartyl protein carboxyl methyltransferase antibody
    • PCMT 1 antibody
    • Pcmt1 antibody
    • PIMT antibody
    • PIMT_HUMAN antibody
    • Protein beta aspartate methyltransferase antibody
    • Protein L isoaspartate (D aspartate) O methyltransferase antibody
    • Protein L isoaspartyl/D aspartyl methyltransferase antibody
    • Protein L-isoaspartyl/D-aspartyl methyltransferase antibody
    • Protein-beta-aspartate methyltransferase antibody
    • Protein-L-isoaspartate(D-aspartate) O-methyltransferase antibody
    see all

Anti-PCMT1 antibody 画像

  • Anti-PCMT1 antibody (ab97446) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 25 kDa
  • Immunohistochemical analysis of PCMT1 in paraffin embedded U87 xenograft, using ab97446 at a 1/500 dilution.

Anti-PCMT1 antibody (ab97446) 使用論文

This product has been referenced in:
  • Yan G  et al. Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1. Int J Cardiol 168:3291-9 (2013). Human . Read more (PubMed: 23647599) »

See 1 Publication for this product

Product Wall

Thank you for your email. I am very sorry it has taken some time to reply, my Spanish colleague Ariana is currently out of the office. The additional blocking step with 0.3% solution of H2O2 in methanol is suitable for HRP detection methods. I would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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