製品の概要

  • 製品名Anti-PCMT1 antibody
    PCMT1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PCMT1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-229 of Human PCMT1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Function: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Similarity: Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • L isoaspartyl protein carboxyl methyltransferase antibody
      • L isoaspartyl/D aspartyl methyltransferase antibody
      • L-isoaspartyl protein carboxyl methyltransferase antibody
      • PCMT 1 antibody
      • Pcmt1 antibody
      • PIMT antibody
      • PIMT_HUMAN antibody
      • Protein beta aspartate methyltransferase antibody
      • Protein L isoaspartate (D aspartate) O methyltransferase antibody
      • Protein L isoaspartyl/D aspartyl methyltransferase antibody
      • Protein L-isoaspartyl/D-aspartyl methyltransferase antibody
      • Protein-beta-aspartate methyltransferase antibody
      • Protein-L-isoaspartate(D-aspartate) O-methyltransferase antibody
      see all

    Anti-PCMT1 antibody 画像



    • Predicted band size : 25 kDa
    • All lanes : Anti-PCMT1 antibody (ab55510) at 1/1000 dilution

      Lane 1 : Whole cell lysates prepared from human 293T cells
      Lane 2 : Whole cell lysate prepared from human Hela cells

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa Fluor® 680 conjugated goat polyclonal to mouse Ig at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 25 kDa


      Exposure time : 2 minutes

      This image is courtesy of an anonymous Abreview

    Anti-PCMT1 antibody (ab55510) 使用論文

    ab55510 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T (lane 1) and Hela (lane 2))
    Loading amount 20 µg
    Specification 293T (lane 1) and Hela (lane 2)
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    投稿 Nov 17 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"