製品の概要

  • 製品名Anti-PCM1 antibody
    PCM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PCM1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • 免疫原

    A synthetic peptide corresponding to a region between residues 1925 and 1975 of human PCM1.

  • ポジティブ・コントロール
    • HeLa whole cell lysate; 293T and mouse NIH3T3 cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 270 kDa.
IP Use a concentration of 2 - 5 µg/ml.

ターゲット情報

  • 機能Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
  • 組織特異性Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
  • 関連疾患Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
    Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1.
  • 配列類似性Belongs to the PCM1 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPCM-1 antibody
    • PCM-1 antibody
    • pcm1 antibody
    • PCM1_HUMAN antibody
    • Pericentriolar material 1 antibody
    • Pericentriolar material 1 protein antibody
    • PTC4 antibody
    see all

Anti-PCM1 antibody 画像

  • All lanes : Anti-PCM1 antibody (ab72443) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg


    Observed band size : 270 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation of PCM1 from 1mg HeLa whole cell lysate by 3 ug ab72443 followed by WB (20% of IP loaded):
    Lane 1: PCM1 precipitate

    Lane 2: Control IgG

    For blotting immunoprecipitated PCM1, ab72443 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-PCM1 antibody (ab72443) 使用論文

This product has been referenced in:
  • Gerdes JM  et al. Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents. Nat Commun 5:5308 (2014). WB . Read more (PubMed: 25374274) »

See 1 Publication for this product

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