製品の概要

  • 製品名Anti-PCK2 antibody
    PCK2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PCK2
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1 - 255 of human PCK2 (AAH01454) with a 26 kDa tag

  • ポジティブ・コントロール
    • A-431 cell lysate; immunogen

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • パスウェイCarbohydrate biosynthesis; gluconeogenesis.
  • 関連疾患Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • 配列類似性Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 4.1.1.32 antibody
    • GTP mitochondrial precursor antibody
    • HGNC:8725 antibody
    • mitochondrial antibody
    • Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody
    • OTTHUMP00000164700 antibody
    • PCK2 antibody
    • PCKGM_HUMAN antibody
    • PE antibody
    • PEP carboxykinase antibody
    • PEPCK antibody
    • PEPCK deficiency mitochondrial antibody
    • PEPCK M antibody
    • PEPCK-M antibody
    • PEPCK2 antibody
    • Phosphoenolpyruvate carboxykinase [GTP] antibody
    • Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody
    • Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

Anti-PCK2 antibody 画像

  • Anti-PCK2 antibody (ab77047) at 1/500 dilution + recombinant tagged PCK2 protein (the immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG(H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 71 kDa
    Observed band size : 96 kDa (why is the actual band size different from the predicted?)
  • Anti-PCK2 antibody (ab77047) at 1/500 dilution + A-431 cell lysate at 25 µg

    Secondary
    goat anti-kouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 71 kDa
    Observed band size : 71 kDa

Anti-PCK2 antibody (ab77047) 使用論文

This product has been referenced in:
  • Leithner K  et al. PCK2 activation mediates an adaptive response to glucose depletion in lung cancer. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24632615) »
  • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). WB ; Human . Read more (PubMed: 21242281) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"