製品の概要

  • 製品名Anti-PCK1 antibody [EPR6939]
    PCK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6939] to PCK1
  • アプリケーション適用あり: WBmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to C terminal amino acids of Human PCK1.

  • ポジティブ・コントロール
    • Human fetal kidney and Human adipose tissue lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

アプリケーション

Our Abpromise guarantee covers the use of ab125003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
    • 組織特異性Major sites of expression are liver, kidney and adipocytes.
    • パスウェイCarbohydrate biosynthesis; gluconeogenesis.
    • 関連疾患Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
    • 配列類似性Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
    • 翻訳後修飾Acetylation is increased on addition of glucose and appears to regulate the protein stability.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • cytosolic [GTP] antibody
      • GTP antibody
      • PCK1 antibody
      • PCKGC_HUMAN antibody
      • PEP carboxykinase antibody
      • PEPCK-C antibody
      • PEPCK1 antibody
      • PEPCKC antibody
      • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
      • Phosphoenolpyruvate carboxykinase 1 antibody
      • Phosphoenolpyruvate carboxykinase antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic antibody
      • Phosphoenolpyruvate carboxylase antibody
      • Phosphopyruvate carboxylase antibody
      see all

    Anti-PCK1 antibody [EPR6939] 画像

    • All lanes : Anti-PCK1 antibody [EPR6939] (ab125003) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human adipose tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-PCK1 antibody [EPR6939] (ab125003) 使用論文

    ab125003 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab125003.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"