製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PCK1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Horse, Chicken, Cow, Pig, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PCK1.

    (Peptide available as ab87372.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human Liver Rat Liver Mouse Liver

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).Can be blocked with PCK1 peptide (ab217264).

ターゲット情報

  • 機能
    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • 組織特異性
    Major sites of expression are liver, kidney and adipocytes.
  • パスウェイ
    Carbohydrate biosynthesis; gluconeogenesis.
  • 関連疾患
    Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • 配列類似性
    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • 翻訳後修飾
    Acetylation is increased on addition of glucose and appears to regulate the protein stability.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cytosolic [GTP] antibody
    • GTP antibody
    • PCK1 antibody
    • PCKGC_HUMAN antibody
    • PEP carboxykinase antibody
    • PEPCK-C antibody
    • PEPCK1 antibody
    • PEPCKC antibody
    • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
    • Phosphoenolpyruvate carboxykinase 1 antibody
    • Phosphoenolpyruvate carboxykinase antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
    • Phosphoenolpyruvate carboxykinase, cytosolic antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

画像

  • All lanes : Anti-PCK1 antibody (ab87340) at 2 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 70 kDa
    Observed band size : 70 kDa


    Exposure time : 15 minutes

プロトコール

参考文献

This product has been referenced in:
  • Wang HY  et al. AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J 449:479-89 (2013). Read more (PubMed: 23078342) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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