Anti-PCK1/PEPC 抗体 (ab87340)
Key features and details
- Rabbit polyclonal to PCK1/PEPC
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PCK1/PEPC antibody
PCK1/PEPC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PCK1/PEPC -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Horse, Chicken, Cow, Pig, Zebrafish, Orangutan -
免疫原
Synthetic peptide corresponding to Human PCK1/PEPC aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab217264) -
ポジティブ・コントロール
- Recombinant Human PCK1/PEPC protein (ab114362) can be used as a positive control in WB. This antibody gave a positive signal in the following tissue lysates: Human Liver Rat Liver Mouse Liver
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87340の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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特記事項 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
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機能
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
組織特異性
Major sites of expression are liver, kidney and adipocytes. -
パスウェイ
Carbohydrate biosynthesis; gluconeogenesis. -
関連疾患
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
配列類似性
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
翻訳後修飾
Acetylation is increased on addition of glucose and appears to regulate the protein stability. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 396458 Chicken
- Entrez Gene: 282855 Cow
- Entrez Gene: 5105 Human
- Entrez Gene: 18534 Mouse
- Entrez Gene: 100144531 Pig
- Entrez Gene: 362282 Rat
- Entrez Gene: 378727 Zebrafish
- Omim: 614168 Human
see all -
別名
- cytosolic [GTP] antibody
- GTP antibody
- PCK1 antibody
see all
画像
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All lanes : Anti-PCK1/PEPC antibody (ab87340) at 2 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 15 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab87340 は 2 報の論文で使用されています。
- Ma X et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). PubMed: 31367261
- Wang HY et al. AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J 449:479-89 (2013). PubMed: 23078342