製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PCK1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Orangutan
  • 免疫原

    Synthetic peptide:

    C-EKEVEDIEKYLEDQ

    , corresponding to internal sequence (near C terminus) amino acids 586-599 of Human PCK1

  • ポジティブ・コントロール
    • Human Kidney lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Peptide ELISA: antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
    • 組織特異性
      Major sites of expression are liver, kidney and adipocytes.
    • パスウェイ
      Carbohydrate biosynthesis; gluconeogenesis.
    • 関連疾患
      Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
    • 配列類似性
      Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
    • 翻訳後修飾
      Acetylation is increased on addition of glucose and appears to regulate the protein stability.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • cytosolic [GTP] antibody
      • GTP antibody
      • PCK1 antibody
      • PCKGC_HUMAN antibody
      • PEP carboxykinase antibody
      • PEPCK-C antibody
      • PEPCK1 antibody
      • PEPCKC antibody
      • Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
      • Phosphoenolpyruvate carboxykinase 1 antibody
      • Phosphoenolpyruvate carboxykinase antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody
      • Phosphoenolpyruvate carboxykinase, cytosolic antibody
      • Phosphoenolpyruvate carboxylase antibody
      • Phosphopyruvate carboxylase antibody
      see all

    画像

    • Anti-PCK1 antibody (ab61701) at 0.5 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer)

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    プロトコール

    参考文献

    This product has been referenced in:
    • Jornayvaz FR  et al. Thyroid hormone receptor-a gene knockout mice are protected from diet-induced hepatic insulin resistance. Endocrinology 153:583-91 (2012). WB ; Mouse . Read more (PubMed: 22147010) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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