Anti-PCK1/PEPC 抗体 (ab115693)
Key features and details
- Goat polyclonal to PCK1/PEPC
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PCK1/PEPC antibody
PCK1/PEPC 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PCK1/PEPC -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis -
免疫原
Synthetic peptide corresponding to Human PCK1/PEPC aa 500-600 (internal sequence) (Cysteine residue).
Database link: NP_002582.3 -
ポジティブ・コントロール
- Human kidney and liver tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115693の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
組織特異性
Major sites of expression are liver, kidney and adipocytes. -
パスウェイ
Carbohydrate biosynthesis; gluconeogenesis. -
関連疾患
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
配列類似性
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
翻訳後修飾
Acetylation is increased on addition of glucose and appears to regulate the protein stability. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 282855 Cow
- Entrez Gene: 403560 Dog
- Entrez Gene: 5105 Human
- Entrez Gene: 18534 Mouse
- Entrez Gene: 100144531 Pig
- Entrez Gene: 362282 Rat
- Entrez Gene: 379844 Xenopus laevis
- Omim: 614168 Human
see all -
別名
- cytosolic [GTP] antibody
- GTP antibody
- PCK1 antibody
see all
画像
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ab115693, at 2µg/ml, staining PCK1//PEPC in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115693, at 2µg/ml, staining PCK1/PEPC in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab115693 は 1 報の論文で使用されています。
- Zhao L et al. PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer. Biomed Pharmacother 118:109251 (2019). PubMed: 31351426