製品の概要

  • 製品名Anti-PCID1 antibody
    PCID1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PCID1
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD, corresponding to N terminal amino acids 1-50 of Human PCID1

  • ポジティブ・コントロール
    • HepG2 cell lysates and Human kidney tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab69842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).
  • 組織特異性Broadly expressed.
  • 配列類似性Belongs to the eIF-3 subunit M family.
    Contains 1 PCI domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B5 antibody
    • B5 receptor antibody
    • Dendritic cell protein antibody
    • eIF3m antibody
    • EIF3M_HUMAN antibody
    • Eukaryotic translation initiation factor 3 subunit M antibody
    • Fetal lung protein B5 antibody
    • FLJ29030 antibody
    • GA17 antibody
    • hfl B5 antibody
    • hFL-B5 antibody
    • PCI domain containing 1 (herpesvirus entry mediator) antibody
    • PCI domain-containing protein 1 antibody
    • PCID1 antibody
    • TANGO7 antibody
    see all

Anti-PCID1 antibody 画像

  • Anti-PCID1 antibody (ab69842) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PCID1 with ab69842 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-PCID1 antibody (ab69842) 使用論文

This product has been referenced in:
  • Cheshenko N  et al. HSV usurps eukaryotic initiation factor 3 subunit M for viral protein translation: novel prevention target. PLoS One 5:e11829 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20676407) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"