Anti-PCDH7 抗体 (ab55506)

製品の概要

  • 製品名Anti-PCDH7 antibody
    PCDH7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PCDH7
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Xenopus laevis, Recombinant Fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant fragment: KQLLRYRLAE EGPADVRIGN VASDLGIVTG SGEVTFSLES GSEYLKIDNL TGELSTSERR IDREKLPQCQ MIFDENECFL DFEVSVIGPS QSWV, corresponding to amino acids 31-125 of Human PCDH7

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性Expressed predominantly in brain and heart and at lower levels in various other tissues.
    • 配列類似性Contains 7 cadherin domains.
    • 翻訳後修飾Isoform C is phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BH Pcdh antibody
      • BH protocadherin antibody
      • BH-Pcdh antibody
      • BHPCDH antibody
      • Brain heart protocadherin antibody
      • Brain-heart protocadherin antibody
      • PCDH 7 antibody
      • PCDH7 antibody
      • PCDH7_HUMAN antibody
      • Protocadherin 7 antibody
      • Protocadherin-7 antibody
      see all

    Anti-PCDH7 antibody 画像

    • Western blot against tagged recombinant protein immunogen using ab55506 PCDH7 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

    Anti-PCDH7 antibody (ab55506) 使用論文

    ab55506 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    Thank you for your reply and the additional information.
    I have one last question:
    Since a truncated PCDH7 construct (+ GFP) was used, I assume the construct contained the antibody binding site (immunogen: KQLLRYRLAE EGPADVRIGN VASDLGIVTG SGE...

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    Thank you for sending the protocol details. This very helpful.
    I have a few additional questions:
    1) At what MW was the band for GFP-PCDH7 seen?
    2) Does the secondary antibody work well with other primaries?
    3) What lysis buffer was...

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    Thank you for contacting us.
    I am sorry to hear the antibody is not working as expected.
    As we discussed over the phone, can you please send me the WB image as well as protocol information you have from your colleagues, so that we can evaluat...

    Read More

    Thank you for your call today.

    As we discussed, please let me know which of these options you would prefer, and I'll be happy to help you further.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Xenopus laevis Tissue lysate - whole (Eye)
    Loading amount 15 µg
    Specification Eye
    Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Verified customer

    投稿 Nov 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"