製品の概要

  • 製品名Anti-PCDH19 antibody
    PCDH19 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PCDH19
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: RLVPRDVEET DKMNVVSCSS LTSSLNYFDY HQQTLPLGCR RSESTFLNVE NQNTRNTSAN HIYHHSFNSQ GPQQPDLIIN GAPLPETENY SFDSNYVNSR , corresponding to amino acids 241-341 of Human PCDH19

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Potential calcium-dependent cell-adhesion protein.
  • 組織特異性Moderately expressed in all regions of the brain examined, with lowest levels found in the cerebellum. Moderate expression is also found in ovary, and low expression in all other tissues tested. Also detected in primary skin fibroblast.
  • 関連疾患Epileptic encephalopathy, early infantile, 9
  • 配列類似性Contains 6 cadherin domains.
  • 発生段階Expressed in developing cortical plate, amygdala and subcortical regions and in the ganglionic eminence.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA1313 antibody
    • PCD19_HUMAN antibody
    • PCDH19 antibody
    • Protocadherin 19 antibody
    • Protocadherin-19 antibody
    see all

Anti-PCDH19 antibody 画像

  • Western blot against tagged recombinant protein immunogen using ab57510 PCDH19 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab57510 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57510, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SH-SY5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-PCDH19 antibody (ab57510) 使用論文

ab57510 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (postnatal day 10 cerebral cortex)
Gel Running Conditions Reduced Denaturing
Loading amount 100 µg
Specification postnatal day 10 cerebral cortex
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Apr 17 2015

Application Immunocytochemistry
Sample Human Cultured Cells (Human PCDH19 transfected in HEK 293FT cell line)
Specification Human PCDH19 transfected in HEK 293FT cell line
Permeabilization Yes - permeabilization was achieved with GDB buffer during ab incubation
Fixative Paraformaldehyde
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投稿 Mar 11 2015

Application Western blot
Loading amount 2 µg
Gel Running Conditions Reduced Denaturing (7,5% gel)
Sample Human Cell lysate - whole cell (Human PCDH19 transfected in HEK 293FT cell line)
Specification Human PCDH19 transfected in HEK 293FT cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Mar 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"