製品の概要

  • 製品名Anti-PCCA antibody
    PCCA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PCCA
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: HVLYYSRQCL MVSRNLGSVG YDPNEKTFDK ILVANRGEIA CRVIRTCKKM, corresponding to amino acids 36-85 of Human PCCA (NP_000273).

  • ポジティブ・コントロール
    • PCCA transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • パスウェイMetabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.
  • 関連疾患Defects in PCCA are the cause of propionic acidemia type I (PA-1) [MIM:606054]. PA-1 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein.
  • 配列類似性Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • mitochondrial antibody
    • Pcca antibody
    • pccA COMPLEMENTATION GROUP antibody
    • PCCA_HUMAN antibody
    • PCCase subunit alpha antibody
    • Propanoyl CoA:carbon dioxide ligase subunit alpha antibody
    • Propanoyl-CoA:carbon dioxide ligase subunit alpha antibody
    • Propionyl CoA carboxylase alpha chain antibody
    • Propionyl CoA carboxylase alpha chain mitochondrial antibody
    • Propionyl CoA carboxylase alpha polypeptide antibody
    • Propionyl coenzyme A carboxylase alpha polypeptide antibody
    • Propionyl-CoA carboxylase alpha chain antibody
    see all

Anti-PCCA antibody 画像

  • Anti-PCCA antibody (ab80346) at 1 µg/ml (in 5% skim milk / PBS buffer) + PCCA transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

Anti-PCCA antibody (ab80346) 使用論文

ab80346 has not yet been referenced specifically in any publications.

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