製品の概要

  • 製品名Anti-PCB antibody [3H2AD9]
    PCB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3H2AD9] to PCB
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IP, In-Cell ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Purified pyruvate carboxylase from bovine liver

  • ポジティブ・コントロール
    • Human fibroblast and HeLa cells; Human liver tissue lysate.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml. For 2 hours.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
  • パスウェイCarbohydrate biosynthesis; gluconeogenesis.
  • 関連疾患Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
  • 配列類似性Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • mitochondrial antibody
    • OTTHUMP00000235155 antibody
    • OTTHUMP00000235156 antibody
    • PC antibody
    • PCB antibody
    • Pcx antibody
    • PYC_HUMAN antibody
    • Pyruvate carboxylase antibody
    • Pyruvate carboxylase mitochondrial antibody
    • Pyruvic carboxylase antibody
    see all

Anti-PCB antibody [3H2AD9] 画像

  • Upper image shows: Immunocytochemistry analysis using ab110314 at 5µg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
    Note: The target protein locates to the mitochondrial matrix.
    Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color.
  • Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate.
  • Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).

Anti-PCB antibody [3H2AD9] (ab110314) 使用論文

ab110314 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"