PC3 whole cell lysate (ab3954)

製品の概要

  • 製品名PC3 whole cell lysate
  • 特記事項Cell line: PC-3 (Human prostate adenocarcinoma).
    Growth media: DMEM/Hams F12 and 10% NCS (Newborn Calf serum).


    PC 3 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーConstituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 精製度Whole Cell Lysate
  • ライゼート 備考PC 3 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab3954 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent concentration. 10 µg to 20 µg per lane is recommended for mini gel.

    PC3 whole cell lysate (ab3954) 使用論文

    ab3954 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email and patience. As stated on the online datasheet for ab19380, the recommended positive control are human PC3 cells (prostate cancer cell line) in a hypoxic state. Ab3954 (PC3 whole cell lysate) was not prepared under hypoxic con...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"