PC12 nuclear extract lysate (hypoxia treated) (ab14886)

製品の概要

  • 製品名
    PC12 nuclear extract lysate (hypoxia treated)
    PC12 ライゼート 製品一覧
  • 製品の詳細
    PC12 nuclear extract lysate
  • 特記事項
    This PC-12 extract (Hypoxia) was tested positively in Western blot and EMSA for NFKB p50 and Pax-3. Before harvesting the cells were deprived of oxygen for 4 hours. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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  • 研究分野
  • 背景
    Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

参考文献

ab14886 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for getting back to us and providing further information. This PC-12 extract (Hypoxia) was tested positively in Western blot and EMSA for NFKB p50 and Pax-3. However, it has not been used to look for HIF-1a or HIF-2a. In terms of trea...

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Thank you for your getting back to us and for providing further information about your experiment. Firstly, we would like to know how this lysate was stored at-80°C in your lab? We would like to emphasize that there is a difference in the way c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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